The car, which was a 2007 Buick LaCrosse, was totaled and the children were pronounced dead at the scene Friday, Missouri State Highway Patrol Sgt. Andy Bell told CNN.
The vehicle struck a guardrail before hitting the ground again and becoming airborne for a second time.
The vehicle then struck a guy-wire — a tensioned cable that is designed to add stability to a freestanding structure — began to overturn, struck a tree and then came to rest off the road on its top before catching fire.
The children were not wearing seat belts.
The accident occurred at approximately 4:20 p.m.; the names of the victims were not released.